Petr Svoboda

The paper shows that a negatively charged intrinsically disordered region (IDR) in Dicer autoinhibits the enzyme and ensures fidelity of miRNA biogenesis.

This study shows that enhanced RNAi in mice does not improve antiviral immunity in vivo, but may work in vitro under specific conditions.

The paper shows that enhancing RNAi in testes via oocyte-specific Dicer does not rescue spermatogenic defects caused by Mili loss.

This study shows that Dicer{Delta}HEL1/wt mice exhibit increased endogenous siRNA levels and functional RNAi in vivo, providing a new model for studying mammalian canonical RNAi.

This study identifies the DExD/H helicase domain of Dicer as critical for accurate miRNA biogenesis, showing its role in substrate recognition, cleavage, and strand selection in mammals.

The paper identifies rare, highly abundant miRNAs in mammalian oocytes that resist dilution and efficiently suppress gene expression, supporting the stoichiometric hypothesis.

This study shows that piRNAs are essential for early embryonic development and spermatogonia establishment in golden hamsters, with distinct effects on male and female fertility.

The paper shows that low miRNA abundance in mammalian oocytes disables the miRNA pathway, as demonstrated by functional assays and cross-species analysis.

The paper investigates the roles of RNAi and piRNA pathways in mouse oocytes, finding that they redundantly target specific retrotransposons but are not essential for oocyte development.

The paper identifies siRNA production as the main constraint for RNAi in mouse cells, showing that Dicer activity, dsRNA structure, and targeting efficiency are critical factors.

This study shows that Cnot6l is essential for maternal mRNA turnover during oocyte-to-embryo transition in mice, with loss of Cnot6l leading to reduced litter size and developmental arrest.