Radek Malı́k

A negatively charged unstructured loop autoinhibits mammalian Dicer and supports fidelity of miRNA biogenesis.

The paper shows that a negatively charged intrinsically disordered region (IDR) in Dicer autoinhibits the enzyme and ensures fidelity of miRNA biogenesis.

Enhanced RNAi does not provide efficient innate antiviral immunity in mice in vivo.

This study shows that enhanced RNAi in mice does not improve antiviral immunity in vivo, but may work in vitro under specific conditions.

Activated RNAi does not rescue piRNA pathway deficiency in testes

The paper shows that enhancing RNAi in testes via oocyte-specific Dicer does not rescue spermatogenic defects caused by Mili loss.

Genetic activation of canonical RNA interference in mice

This study shows that Dicer{Delta}HEL1/wt mice exhibit increased endogenous siRNA levels and functional RNAi in vivo, providing a new model for studying mammalian canonical RNAi.

Molecular basis of indispensable accuracy of mammalian miRNA biogenesis

This study identifies the DExD/H helicase domain of Dicer as critical for accurate miRNA biogenesis, showing its role in substrate recognition, cleavage, and strand selection in mammals.

Golden hamster piRNAs are necessary for early embryonic development and establishment of spermatogonia

This study shows that piRNAs are essential for early embryonic development and spermatogonia establishment in golden hamsters, with distinct effects on male and female fertility.

Low miRNA abundance disables microRNA pathway in mammalian oocytes

The paper shows that low miRNA abundance in mammalian oocytes disables the miRNA pathway, as demonstrated by functional assays and cross-species analysis.

Restricted and non-essential redundancy of RNAi and piRNA pathways in mouse oocytes

The paper investigates the roles of RNAi and piRNA pathways in mouse oocytes, finding that they redundantly target specific retrotransposons but are not essential for oocyte development.

Main constraints for RNAi induced by expressed long dsRNA in mouse cells

The paper identifies siRNA production as the main constraint for RNAi in mouse cells, showing that Dicer activity, dsRNA structure, and targeting efficiency are critical factors.

Role of Cnot6l in maternal mRNA turnover

This study shows that Cnot6l is essential for maternal mRNA turnover during oocyte-to-embryo transition in mice, with loss of Cnot6l leading to reduced litter size and developmental arrest.