Validation and Long-Term Follow Up of CD33 Off-Targets Predicted In Vitro and In Silico Using Error-Corrected Sequencing in Rhesus Macaques
Aisha A. Aljanahi, Cícera Regina Lazzarotto, Shirley Chen, Tae–Hoon Shin, Stefan Cordes, Isabel Jabara, Yifan Zhou, David J. Young, Byung‐Chul Lee, Kyung–Rok Yu, Yuesheng Li, Bradley S. Toms, Ilker Tunc, So Gun Hong, Lauren L. Truitt, , Cynthia E. Dunbar
The paper validates off-target effects of CRISPR/Cas9 in rhesus macaques using error-corrected sequencing, showing that in vitro and in silico methods have limited predictive accuracy.
bioRxiv · Jul 5, 2020 · PreprintMolecular Biology