Cesare Orlandi

This study predicts 3D structures of human GPCR-G protein complexes using AlphaFold3 and uses machine learning to predict coupling potency, revealing new signaling mechanisms and coupling preferences across GPCR subtypes.

The paper identifies NA-Gly, DHA-Gly, and their epoxide derivatives as multifunctional lipid mediators with anti-inflammatory properties and selective receptor modulation, suggesting therapeutic potential for neuroinflammation.

The paper investigates the G protein coupling profile of dopamine D3 receptor, finding it selectively activates Go and Gz subtypes but not Gi proteins, with structural determinants identified.

GZESTY is a cell-based assay for Gi/o/z-coupled GPCRs that detects ligand activation and identifies endogenous ligands for orphan GPCRs.