Sarra Merzouk

The paper presents a guide selection strategy to optimize Cas9 CRISPR knockout library design by balancing on-target and off-target activity, resulting in an updated validated library for the human genome.

This study designs an optimized Cas9 CRISPRi library by improving on-target scoring and analyzing off-target effects using updated datasets and methods.

The paper shows that X-chromosome upregulation occurs gene-by-gene at both RNA and protein levels in mouse pluripotent stem cells.

This study identifies HCFC1 and YY1 as factors that recruit COMPASS and Integrator complexes to initiate X chromosome inactivation by regulating Xist and its cis-regulatory elements.

This paper introduces two mouse embryoid models to study interactions between primitive endoderm and epiblast during early development, showing they replicate in vivo dynamics and cell fate decisions.

This study shows that SPEN is essential for Xist upregulation during X chromosome inactivation initiation in female mouse ESCs by silencing Tsix.