Author

Smriti Srikanth

Broad InstituteORCID 0009-0003-5719-8433Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Assay:CRISPR screen· 3 Organism:human· 2 Approach:statistical model· 2 Research Focus:benchmarking· 1 Approach:deep learning· 1 Research Focus:drug response / drug discovery· 1 Approach:experimental assay· 1 Research Focus:perturbation response prediction· 1

Frequent Co-Authors

John G. DoenchiD· 3 shared Laura M. DrepanosiD· 3 shared Berta Escude Velasco· 2 shared Sarra MerzoukiD· 2 shared Spencer T. ShahiD· 2 shared Abigail Chase· 1 shared Allison Uebele· 1 shared Andrew W. NaviaiD· 1 shared

Activity by Year

2026-2026 · peak 3/yr

Co-Author Collaboration Network

10 top collaborators · 31 mutual co-author links

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3 shared papersGenomics

John G. DoenchORCID

Mutual co-authors in cluster

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3 papers

Recent·Most cited

GenomicsbioRxivPreprintJul 4, 2026

Direct comparison of CRISPR knockout and interference with Perturb-seq

Laura M. Drepanos, Berta Escude Velasco, Abigail Chase…·John G. Doench

This study compares CRISPR knockout and interference using Perturb-seq, finding consistent transcriptional signatures and tradeoffs in off-target activity and cellular response artifacts.

DOI 10.64898/2026.07.04.736492

CRISPR screenexperimental assay

𝕏 PostBluesky

GenomicsCell GenomicsPeer-reviewedMar 25, 2026

Balancing off-target and on-target considerations for optimized Cas9 CRISPR knockout library design

Laura M. Drepanos, Smriti Srikanth, Eleanor G. Kaplan…·John G. Doench

The paper presents a guide selection strategy to optimize Cas9 CRISPR knockout library design by balancing on-target and off-target activity, resulting in an updated validated library for the human genome.

9 citationstop 1%

humanCRISPR screen

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BioengineeringbioRxivPreprintFeb 26, 2026

Optimized parameters for Cas9 CRISPR interference library design

Smriti Srikanth, Fengyi Zheng, Laura M. Drepanos…·John G. Doench

This study designs an optimized Cas9 CRISPRi library by improving on-target scoring and analyzing off-target effects using updated datasets and methods.

2 citations

humanCRISPR screen

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