Laura M. Drepanos

This study compares CRISPR knockout and interference using Perturb-seq, finding consistent transcriptional signatures and tradeoffs in off-target activity and cellular response artifacts.

This study identifies KDM6 enzymes as targets of R2HG from mutant IDH, linking them to replication stress sensitivity in gliomas and introduces a DHODH inhibitor, GLIO-1, with potential clinical applications.

The paper presents a guide selection strategy to optimize Cas9 CRISPR knockout library design by balancing on-target and off-target activity, resulting in an updated validated library for the human genome.

This study designs an optimized Cas9 CRISPRi library by improving on-target scoring and analyzing off-target effects using updated datasets and methods.

This paper introduces an activity-based selection method to improve base editing efficiency and precision in identifying functional mutations.