Amplification-free long read sequencing reveals unforeseen CRISPR-Cas9 off-target activity
Ida Höijer, Josefin Johansson, Sanna Gudmundsson, Chen-Shan Chin, Ignas Bunikis, Susana Häggqvist, Anastasia Emmanouilidou, Maria Wilbe, Marcel den Hoed, Marie-Louise Bondeson, Lars Feuk, Ulf B Gyllensten, Adam Ameur
The paper introduces amplification-free long-read sequencing protocols to detect CRISPR-Cas9 off-target binding sites, identifying many previously undetected sites that are not predicted by existing software.
Genome biology · Dec 1, 2020Peer-reviewedGenomics