Francisco J. M. Mojica

This paper characterizes DO1Cas9, a cold-adapted Cas9 nuclease from the deep ocean, showing improved activity under cold conditions and broader organismal applicability.

The paper identifies a high abundance of CRISPR-Cas systems in deep ocean microbiomes, highlighting their diversity and potential for bioprospecting.

The paper identifies a new CRISPR-Cas9 system from a metagenome, characterized by a smaller Cas9 protein (EHCas9) with DNA targeting capabilities, and develops a simplified tool for genome editing in prokaryotes and eukaryotic cells.