Aging, dauer, and stature phenotypes are conferred by structure-directed missense mutations in the endogenous AGE-1/phosphatidylinositol 3-kinase catalytic subunit.
You Wu, Tam Duong, Neal R. Rasmussen…·David J. Reiner
The paper uses structure-guided CRISPR/Cas9 to study PI3K mutations in C. elegans, revealing a novel Ras>PI3K signaling axis that affects growth, dauer formation, and lifespan.