CRISPR/Cas9 genome editing to generate single variant Plasmodium falciparum lines and enable reverse genetic studies of PfEMP1 function in live parasites
Stanley E Otoboh, Hussein Molu Abkallo, Jessie Jungels…·J Alexandra Rowe
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Nagasaki UniversityORCID 0000-0002-5594-4418Unclaimed ORCID profile · follow to get notified of new preprints
3 papers
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Stanley E Otoboh, Hussein Molu Abkallo, Jessie Jungels…·J Alexandra Rowe
Amit Kumar Subudhi, Aidan J. O’Donnell, Abhinay Ramaprasad…·Arnab Pain
The paper shows that malaria parasites' intraerythrocytic cycle duration is influenced by coordination with host circadian rhythms, with gene expression and pathways affected when this coordination is disrupted.
Hussein Molu Abkallo, Axel Martinelli, Megumi Inoue…·Richard Leighton Culleton
This paper introduces a method to identify genetic drivers of multiple phenotypes in malaria parasites by combining LGS, sequencing, and mathematical modeling, demonstrating its effectiveness in detecting novel alleles for growth rate and strain-specific immunity.