The two key substitutions in the chromophore environment of mKate2 to produce an enhanced FusionRed-like red fluorescent protein
Dmitry A. Ruchkin, Alexey S. Gavrikov, Danila V. Kolesov…·Alexey M. Bogdanov
The paper describes a new red fluorescent protein variant that outperforms its parents in brightness, fluorescence lifetime, and blinking properties.