Daisuke Saito

The paper shows chick primordial germ cells use a common bleb-based migratory program across different embryonic environments, with distinct molecular mechanisms in each context.

The paper identifies ENS-1/ERNI and its LTR Soprano as factors influencing chicken primordial germ cell development and in vitro establishment efficiency.

This study identifies dCBP as a regulator of histone lactylation in Drosophila meiotic chromatin, showing its role in chromosome maintenance and linking energy metabolism to chromatin regulation.

The paper optimizes ATAC-seq for low-input and cryopreserved chicken primordial germ cells, identifying PGC-specific chromatin regions and their functional roles.

This study compares transcriptomes of cultured and in vivo chicken primordial germ cells using single-cell RNA sequencing, revealing shifts in gene regulatory networks and new intermediate cell states.

The paper shows that all extravasating cells form Ca²⁺-dependent membrane blebs, but the source of Ca²⁺ varies by lineage, highlighting distinct regulatory mechanisms.

The paper shows that repressive SAM biosynthesis in Drosophila germline inhibits HeT-A retrotransposon transcription via 6mA methylation.

The paper uses the yolk sac vasculature of early avian embryos to study cancer cell extravasation, showing distinct morphologies and dynamics through fluorescent labeling.

This paper introduces a method using Lycopersicon Esculentum lectin to label and sort primordial germ cells in avian species, showing high efficiency and subtype identification.

The paper shows that SOCE-mediated Ca2+ elevation drives membrane bleb formation in avian PGCs, essential for in vivo migration.

This study shows that the stiffness of primordial germ cells is required for their arrest and extravasation in avian embryos, with actin polymerization regulating this stiffness.

This study describes a method to apply prime editing to chicken fibroblasts and primordial germ cells, enabling precise gene modification in these cells.