Christian J. G. Tessier

An extended structure of the intracellular domain of the Torpedo nicotinic acetylcholine receptor and its proposed interactions with rapsyn

The paper refines a cryo-EM structure of the Torpedo nAChR intracellular domain and uses it to evaluate AlphaFold3 models, revealing a 3:1 rapsyn binding architecture and its role in receptor clustering.

Asynchronous subunit transitions precede acetylcholine receptor activation

The paper uses cryo-EM and single-channel recordings to show that acetylcholine receptor subunits transition asynchronously, leading to a sequential activation mechanism.

Heteropentameric architecture predisposes functional inequivalence of acetylcholine receptor agonist sites

This study shows that structural asymmetry in acetylcholine receptors causes functional inequivalence between chemically identical agonist sites, highlighting its role in disease and evolution.

pUdOs: concise plasmids for bacterial and mammalian cells

The paper introduces pUdOs, a set of small plasmids with flexible replication and selection markers, useful for bacterial and mammalian cells.