Yan Sun

Rational Design of 5' UTRs for Optimized mRNA Expression and Cost-Effective, Rapid Analysis of Capped mRNA

This paper designs a 5' UTR that enables rapid, cost-effective analysis of mRNA capping efficiency and poly(A) tail length without probes or biotin.

An Engineered Halotolerant Chimeric T7 RNA Polymerase for High-Yield, Low-Immunogenicity Synthesis of RNA via Simple Batch Transcription

This paper introduces a halotolerant chimeric T7 RNA polymerase that improves RNA synthesis yield, reduces immunogenicity, and enhances transcript quality under high-salt conditions.

Application of a High-Biomimetic Tumor Organoid-CAF Co-Culture Model for the Efficacy Evaluation of CAR-T Drugs

This paper develops a tumor organoid-CAF co-culture model to evaluate CAR-T drug efficacy by mimicking the tumor microenvironment's complexity.

Characterizing sensitivity and coverage of clinical WGS as a diagnostic test for genetic disorders

This study evaluates the sensitivity and coverage of clinical whole-genome sequencing (WGS) for detecting genetic variants, finding that 40X mean depth achieves high sensitivity for SNVs and indels but not 100%.

Performance characterization of PCR-free whole genome sequencing for clinical diagnosis

This study evaluates PCR-free whole genome sequencing for clinical diagnosis, showing improved sensitivity and PPV for variant detection compared to PCR-based methods.

Clinical diagnosis of single-gene disorders and chromosomal abnormalities based on BGISEQ-500 platform

This study develops a chip-based sequencing method to detect monogenic diseases and chromosomal abnormalities, identifying 102 mutations in 63 patients with 69 new mutations.

Identification of TTN as a novel candidate gene for atrioventricular block in a Chinese pedigree by whole-exome sequencing

This study identifies a novel TTN mutation causing autosomal dominant atrioventricular block in a Chinese family through whole-exome sequencing and co-segregation analysis.