Regan J. Hayward

High levels of antisense transcription from numerous genes in the obligate intracellular bacterium Orientia tsutsugamushi.

The paper confirms high levels of antisense transcription in Orientia tsutsugamushi across multiple strains and suggests it may be a regulated mechanism in its lifecycle.

Comparative Dual RNA-Seq Analysis of Eight Orientia tsutsugamushi Strains in Endothelial Cells Reveals Regulatory Patterns in Bacterial and Host Pathways During Infection

The paper compares dual RNA-seq data from eight Orientia tsutsugamushi strains in endothelial cells, finding no clear gene expression patterns correlating with virulence but identifying strain-specific interferon responses and conserved bacterial pathways.

An integrated transcriptomics-functional genomics approach reveals a small RNA that modulates Bacteroides thetaiotaomicron sensitivity to tetracyclines

The paper identifies a small RNA, MasB, that increases Bacteroides thetaiotaomicron tolerance to tetracyclines by modulating gene expression under antibiotic stress.

Dual RNA-Seq analysis of in vitro infection multiplicity in Chlamydia-infected epithelial cells

The paper uses dual RNA-seq to analyze transcriptomic responses in Chlamydia-infected epithelial cells across different infection conditions and RNA depletion methods.

Chromatin accessibility dynamics of Chlamydia-infected epithelial cells

This study uses FAIRE-Seq to analyze chromatin accessibility changes in human epithelial cells infected with Chlamydia trachomatis over its developmental cycle, identifying differentially accessible regions linked to immune and metabolic responses.

Early transcriptional landscapes of Chlamydia trachomatis-infected epithelial cells at single cell resolution

The paper uses scRNA-Seq to analyze early host cell responses to Chlamydia trachomatis infection, identifying distinct transcriptional patterns and potential biomarkers.

Dual RNA sequencing (dRNA-Seq) of bacteria and their host cells

This paper introduces a method to simultaneously analyze host and bacterial transcriptomes in infected cells and provides a protocol for its implementation.