Author

Riley D. Metcalfe

National Cancer InstituteORCID 0000-0002-7154-780XUnclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Biochemistry· 1 Immunology· 1 Molecular Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Organism:human· 2 Research Focus:drug response / drug discovery· 1 Research Focus:host-pathogen & immune response· 1 Organism:mouse· 1 Research Focus:protein structure prediction· 1 Assay:structural biology· 1

Frequent Co-Authors

Juliana A. Martinez FiescoiD· 2 shared Mark CooksoniD· 2 shared Ping ZhangiD· 2 shared Alexandra G. BeilinaiD· 1 shared Anandani NellaniD· 1 shared Astrid Alvarez de la CruziD· 1 shared Brad St. CroixiD· 1 shared Dan Li· 1 shared

Activity by Year

2023-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 20 mutual co-author links

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2 shared papersBiochemistry

Juliana A. Martinez FiescoORCID

Mutual co-authors in cluster

Author profile →

3 papers

Recent·Most cited

Engineering Functionality-Optimized Fully Human B7-H3 CAR T Cells for Enhanced Solid Tumor Therapy

Pradip Bajgain, Yang Feng, Mariela Puebla, Meijie Tian, Kuo-Sheng Hsu, Jaewon Lee, Guojun Yu, Liping Yang, Steven Seaman, Mary Beth Hilton, Karen Morris, Niza Borchin, Jennifer D. Tran, Riley D. Metcalfe, Dan Li, , Brad St. Croix

This study develops fully human B7-H3 CAR T cells using phage display, showing improved antitumor activity and reduced immune response in xenograft models.

Cell Reports Medicine · May 1, 2026Peer-reviewedImmunology

14-3-3 binding maintains the Parkinson's associated kinase LRRK2 in an inactive state

Juliana A. Martinez Fiesco, Alexandra G. Beilina, Astrid Alvarez de la Cruz, Ning Li, Riley D. Metcalfe, Mark Cookson, Ping Zhang

The paper presents the cryo-EM structure of the LRRK2:14-3-32 complex, showing how 14-3-3 binding inhibits LRRK2 activity and how PD mutations disrupt this interaction.

Nature Communications · Aug 5, 2025Peer-reviewedBiochemistry

Structure and regulation of full-length human leucine-rich repeat kinase 1

Riley D. Metcalfe, Juliana A. Martinez Fiesco, Luis Bonet‐Ponce, Jillian H. Kluss, Mark Cookson, Ping Zhang

This study reports the cryo-EM structure of LRRK1 and its dimer, revealing structural differences from LRRK2 and providing insights into their regulation.

Nature Communications · Aug 9, 2023Peer-reviewedMolecular Biology