Author

Behrad Shaghaghi

Radboud University NijmegenORCID 0000-0002-7259-6814Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Research Focus:cancer & tumor microenvironment· 1 Research Focus:cell signaling & trafficking· 1 Research Focus:cell type annotation· 1 Organism:human· 1 Assay:proteomics· 1

Frequent Co-Authors

Ana Montero‐CalleiD· 2 shared Charlotte CresensiD· 2 shared Lotte GerritsiD· 2 shared Paul H. J. KouweriD· 2 shared Rodrigo BarderasiD· 2 shared Samet AytekiniD· 2 shared Susana RochaiD· 2 shared Guillermo Solís‐FernándeziD· 1 shared

Activity by Year

2026-2026 · peak 2/yr

Co-Author Collaboration Network

8 top collaborators · 28 mutual co-author links

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2 shared papersBioengineering

Ana Montero‐CalleORCID

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2 papers

Recent·Most cited

Synthesis and mechanical characterization of polyacrylamide (PAAm) hydrogels with different stiffnesses for large-batch cell culture applications

Charlotte Cresens, Samet Aytekin, Behrad Shaghaghi, Lotte Gerrits, Ana Montero‐Calle, Rodrigo Barderas, Paul H. J. Kouwer, Susana Rocha

This paper provides a step-by-step protocol for synthesizing and characterizing PAAm hydrogels of varying stiffness for cell culture and mechanobiology studies.

STAR Protocols · Jun 1, 2026Peer-reviewedBioengineering

Deciphering stiffness-driven changes in colorectal cancer by proteomics

Charlotte Cresens, Ana Montero‐Calle, Guillermo Solís‐Fernández, Behrad Shaghaghi, Lotte Gerrits, Samet Aytekin, Paul H. J. Kouwer, Rodrigo Barderas, Susana Rocha

This study shows that matrix stiffness in colorectal cancer alters secreted protein expression, enhancing migration, angiogenesis, and matrix remodeling, contributing to a more aggressive cancer phenotype.

Molecular & Cellular Proteomics · Jan 24, 2026Peer-reviewedCancer Biology