Bart Claushuis

Elucidation of the glycan structure of the b-type flagellin of Pseudomonas aeruginosa PAO1

This study identifies the unknown moiety in the b-type flagellin glycan of Pseudomonas aeruginosa PAO1 as N,N-dimethylthreonine using mass spectrometry and compares it to Type A glycan in C. difficile.

Non-prime- and Prime-side Profiling of Pro-Pro Endopeptidase Specificity Using Synthetic Combinatorial Peptide Libraries and Mass Spectrometry

The paper characterizes the non-prime- and prime-side specificity of Pro-Pro endopeptidases using synthetic peptide libraries and mass spectrometry, identifying novel PPEPs and altered specificity in mutants.

Proteolytic activity of surface exposed HtrA determines its expression level and is needed to survive acidic conditions in Clostridioides difficile

This study shows that HtrA proteolytic activity affects its expression level and is necessary for C. difficile survival under acidic conditions.