Takayuki Kato

A novel periplasmic layer formed by an outer membrane lipo-protein governs the cell-envelope integrity and stiffness of Leptospira interrogans

This study identifies LipL32 as a key structural protein in Leptospira interrogans that maintains cell-envelope integrity and stiffness, affecting pathogenicity.

Structural and Dynamic Insights into the Biased Signaling Mechanism of the Human Kappa Opioid Receptor

This study investigates the structural dynamics of the human KOR-Gi signaling complex with G-protein-biased and balanced agonists, identifying key residues involved in signaling bias.

The Na+-pumping mechanism driven by redox reactions in the NADH-quinone oxidoreductase from Vibrio cholerae relies on dynamic conformational changes

This study identifies five conformational states of Na+-NQR in Vibrio cholerae using mutants and inhibitors, revealing that 2Fe-2S reduction drives Na+ translocation through structural rearrangements.

Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics

The paper uses optogenetics and cryo-ET to visualize actin cytoskeleton dynamics during lamellipodia formation, revealing distinct structural phases and actin turnover mechanisms.

Dimeric assembly of F1-like ATPase for the gliding motility of Mycoplasma

The paper presents a 3.2 Å resolution cryo-EM structure of a dimeric F1-like ATPase complex in Mycoplasma mobile, revealing its role in gliding motility through conserved rotary mechanisms.

Structural insight into sodium ion pathway in the bacterial flagellar stator from marine Vibrio

The paper determines the structure of the Na+-driven stator PomAB from Vibrio using cryo-EM and reveals the sodium ion pathway, ion selectivity mechanism, and inhibition by phenamil.

cryoMDM; Molecular simulation Driven structural Matching Approach to Estimate Variety of Atomic Three-dimensional Model Based on Noisy cryoEM Single Particle Images.

The paper introduces cryoMDM, a method that estimates 3D atomic models from single cryoEM particle images by matching them to simulated structural models, revealing intermediate structures of the SARS-CoV-2 spike protein.

Fc-independent SARS-CoV-2 infection-enhancing antibodies decouple N-terminal and receptor-binding domains by cross-linking neighboring spikes

This study shows that certain SARS-CoV-2 antibodies enhance infection by cross-linking spike proteins, causing NTD-RBD domain decoupling without needing the Fc region.

Cryo-EM inspired NMR analysis reveals a pH-induced conformational switching mechanism for imparting dynamics to Betanodavirus protrusions

This study reveals a pH-induced conformational switching mechanism in Betanodavirus protrusions that governs infectivity through dynamic structural changes.

Molecular basis of RNase I-mediated rRNA degradation regulated by ribosomes

The paper reveals how hibernating ribosomes inhibit RNase I activity to regulate rRNA degradation and biofilm formation in E. coli.

Epoxidized graphene grid for high-throughput high-resolution cryoEM structural analysis

The paper introduces an epoxidized graphene grid (EG-grid) that improves particle density and orientation in cryoEM, enabling high-resolution structural analysis with fewer micrographs.

Structure of the molecular bushing of the bacterial flagellar motor

The paper reports the structure of the LP ring of the bacterial flagellar motor using electron cryomicroscopy, revealing its 26-fold symmetry and intersubunit interactions.

Extraction of Protein Dynamics Information Hidden in Cryo-EM Map Using Deep Learning

This paper introduces DEFMap, a deep learning method that extracts dynamics information from cryo-EM maps, achieving results comparable to molecular dynamics simulations and experimental approaches.

Sub-3 A resolution structure of apoferritin using a multi-purpose TEM with a side-entry cryo-holder

This study demonstrates that a multi-purpose TEM with a side-entry cryo-holder can achieve high-resolution cryo-EM single particle analysis, as shown by a 3 Å resolution map of apoferritin.