The paper identifies transcriptomic signatures that explain most of the variation in recombinant protein yield in CHO cells producing 2165 human secretome proteins.

bioRxiv · Dec 12, 2022 · PreprintSystems Biology

Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteins

Magdalena Malm, Chih‐Chung Kuo, Mona Moradi Barzadd, Aman Mebrahtu, Num Wistbacka, Ronia Razavi, Anna-Luisa Volk, Magnus Lundqvist, David Kotol, Hanna Tegel, Sophia Hober, Fredrik Edfors, Torbjörn Gräslund, Véronique Chotteau, Ray P. Field, , Johan Rockberg

The paper identifies secretory pathway differences between HEK293 and CHO cells that improve recombinant protein expression when switching hosts.

Metabolic Engineering · Mar 14, 2022Peer-reviewedSystems Biology

Evolution from adherent to suspension - systems biology of HEK293 cell line development

Magdalena Malm, Rasool Saghaleyni, Magnus Lundqvist, Marco Giudici, Véronique Chotteau, Ray P. Field, Paul Varley, Diane Hatton, Luigi Grassi, Thomas Svensson, Mathias Uhlén, Jens Nielsen, Johan Rockberg

The paper analyzes genomic, transcriptomic, and metabolic changes in HEK293 cell lines to understand genetic and compositional differences between adherent and suspension derivatives.

Scientific Reports · Nov 4, 2020Peer-reviewedSystems Biology

The human secretome - the proteins secreted from human cells

Mathias Uhlén, Hanna Tegel, Åsa Sivertsson, Chih‐Chung Kuo, Jahir M. Gutierrez, Nathan E. Lewis, Björn Forsström, Melanie Dannemeyer, Linn Fagerberg, Magdalena Malm, Helian Vunk, Fredrik Edfors, Andreas Hober, Evelina Sjöstedt, David Kotol, , Sophia Hober

The paper maps and characterizes the human secretome by annotating 2,623 predicted secreted proteins and their spatial distribution, using a high-throughput cell factory for recombinant protein production and analysis.

bioRxiv · Nov 27, 2018 · PreprintMolecular Biology