Chih‐Chung Kuo

The paper presents secRecon, a comprehensive secretory pathway reconstruction, and uses it to identify secretory differences in plasma cells and CHO cells, highlighting potential targets for improving secretion capacity.

The paper introduces FcBAR, a method to detect protein-protein interactions in situ for recombinant proteins, and identifies factors that enhance rituximab production.

The paper identifies transcriptomic signatures that explain most of the variation in recombinant protein yield in CHO cells producing 2165 human secretome proteins.

The paper identifies secretory pathway differences between HEK293 and CHO cells that improve recombinant protein expression when switching hosts.

The paper connects Alzheimer's disease genetics to amyloid formation by analyzing secretory pathway dysregulation in AD brains.

The paper identifies protein-protein interactions supporting recombinant protein secretion using BioID and Bayesian modeling, finding key roles for oxidative folding enzymes in disulfide bond formation.

This study improves context-specific metabolic models by integrating data-inferred cell functions, reducing model variability across algorithms and better capturing biological variability.

The paper maps and characterizes the human secretome by annotating 2,623 predicted secreted proteins and their spatial distribution, using a high-throughput cell factory for recombinant protein production and analysis.