Diane Hatton

DNA Damage Driven Viability Loss and Transcriptional Reprogramming in Chinese Hamster Ovary Cell Perfusion Culture

The paper identifies DNA damage accumulation as a key factor limiting viability in Chinese hamster ovary cells during perfusion culture, showing impaired DNA damage response and transcriptional dysfunction.

Identification of Cellular Signatures Associated with Chinese Hamster Ovary (CHO) Cell Adaptation for Secretion of Antibodies

This study identifies cellular adaptations in CHO cells linked to antibody production, finding that transgene copy number and mRNA expression levels are key factors in productivity.

Deciphering the determinants of recombinant protein yield across the human secretome

The paper identifies transcriptomic signatures that explain most of the variation in recombinant protein yield in CHO cells producing 2165 human secretome proteins.

Transcriptome analysis of EPO and GFP HEK293 Cell-lines Reveal Shifts in Energy and ER Capacity Support Improved Erythropoietin Production in HEK293F Cells

The paper compares transcriptomic profiles of HEK293 cell lines producing erythropoietin or GFP to identify factors associated with improved erythropoietin production.

Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteins

The paper identifies secretory pathway differences between HEK293 and CHO cells that improve recombinant protein expression when switching hosts.

Evolution from adherent to suspension - systems biology of HEK293 cell line development

The paper analyzes genomic, transcriptomic, and metabolic changes in HEK293 cell lines to understand genetic and compositional differences between adherent and suspension derivatives.

The human secretome - the proteins secreted from human cells

The paper maps and characterizes the human secretome by annotating 2,623 predicted secreted proteins and their spatial distribution, using a high-throughput cell factory for recombinant protein production and analysis.