Author

Manish Kumar Gupta

Johns Hopkins UniversityORCID 0000-0002-8014-1537Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Research Focus:cell type annotation· 2 Research Focus:drug response / drug discovery· 2 Approach:experimental assay· 2 Assay:proteomics· 2 Organism:bacteria (other)· 1 Assay:bulk RNA-seq· 1 Research Focus:differential expression· 1 Research Focus:gene regulatory network inference· 1

Frequent Co-Authors

Benjamin N. KoleskeiD· 2 shared Courtney SchilliD· 2 shared Rajagopalan SaranathaniD· 2 shared William R. BishaiiD· 2 shared William R. JacobsiD· 2 shared Yazmin B. Martinez-MartineziD· 2 shared Catherine VilchèzeiD· 1 shared Jessica SheniD· 1 shared

Activity by Year

2025-2025 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 42 mutual co-author links

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2 shared papersMicrobiology

Benjamin N. KoleskeORCID

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2 papers

Recent·Most cited

Loss of the ESX-5 secretion locus in Mycobacterium tuberculosis reshapes the mycomembrane and enhances ESX-1 substrate secretion

Benjamin N. Koleske, Rajagopalan Saranathan, Courtney Schill, Shichun Lun, Catherine Vilchèze, Lahari Das, Manish Kumar Gupta, Yazmin B. Martinez-Martinez, William R. Bishai, William R. Jacobs

This study shows that deleting the ESX-5 secretion system in Mycobacterium tuberculosis reduces ESX-5 substrate secretion, increases ESX-1 substrates, decreases ESX-3 expression, and alters cell morphology and antibiotic susceptibility.

Proceedings of the National Academy of Sciences · Sep 3, 2025Peer-reviewedMicrobiology

Loss of the PPE71-esxX-esxY-PPE38 locus drives adaptive transcriptional responses and hypervirulence of Mycobacterium tuberculosis Lineage 2

Benjamin N. Koleske, Courtney Schill, Rajagopalan Saranathan, Somnath Shee, Yazmin B. Martinez-Martinez, Manish Kumar Gupta, Jessica Shen, William R. Jacobs, William R. Bishai

This study shows that loss of the PPE71-38 locus in Mycobacterium tuberculosis Lineage 2 increases stress response gene expression and triglyceride levels, contributing to hypervirulence.

Science Advances · Jul 2, 2025Peer-reviewedMicrobiology