Adam T. Watson

Single-molecule sequencing maps replication dynamics across the fission yeast genome, including centromeres

This paper introduces DNAscent, a nanopore sequencing method to map DNA replication dynamics in fission yeast at single-molecule resolution, revealing replication patterns in centromeres and rDNA.

S. pombe Rtf2 is Important for Replication Fork Barrier Activity of RTS1 via Splicing of Rtf1

The paper shows that Rtf2 in S. pombe is essential for proper splicing of Rtf1, which is necessary for RTS1 barrier activity.

Inhibition of MRN activity by a telomere protein motif

The paper shows that the MIN motif in Rif2 inhibits MRN complex activity at telomeres, preventing DDR activation and NHEJ in yeast.

Live-cell single-molecule tracking highlights requirements for stable Smc5/6 chromatin association in vivo

The paper uses live-cell single-molecule tracking in fission yeast to show that Smc5/6 chromatin association depends on Nse6 and specific sub-pathways, with implications for homologous recombination.

Replication dynamics of recombination-dependent replication forks

This study investigates HR-restart of replication forks using polymerase usage sequencing and Monte Carlo simulations in S. pombe, showing Pol δ synthesizes both strands without maturing to δ/ε and demonstrating uninterrupted fork progression through barriers.