Sathish Thiyagarajan

The paper identifies replication pause sites in a eukaryotic genome using long-read nanopore sequencing and finds they are enriched near transcribed regions and correlate with transcription and R-loop levels.

This paper introduces DNAscent, a nanopore sequencing method to map DNA replication dynamics in fission yeast at single-molecule resolution, revealing replication patterns in centromeres and rDNA.

The paper uses single-molecule sequencing to show most DNA replication initiation in human cells occurs at dispersed sites, not just previously identified zones.

This study shows that deleting the actin binding domain of Leishmania profilin impairs cell growth, vesicle trafficking, and mitochondrial function.