Antony M. Carr

This paper introduces DNAscent, a nanopore sequencing method to map DNA replication dynamics in fission yeast at single-molecule resolution, revealing replication patterns in centromeres and rDNA.

The paper investigates how SUMO protease and proteasome recruitment at nuclear pores affects replication dynamics at arrested forks in fission yeast.

The paper shows that Rtf2 in S. pombe is essential for proper splicing of Rtf1, which is necessary for RTS1 barrier activity.

The paper uses Pu-seq to map DNA polymerase usage in human cells, revealing Polε synthesizes the leading strand and Pol contributes to lagging strand synthesis, and links transcription to polymerase uncoupling and chromosomal instability.

The paper predicts cell line-specific acquired essential genes using perturbed PPI networks and machine learning, achieving AUC ROC scores of 0.75-0.85.

The paper shows that the MIN motif in Rif2 inhibits MRN complex activity at telomeres, preventing DDR activation and NHEJ in yeast.

The paper uses live-cell single-molecule tracking in fission yeast to show that Smc5/6 chromatin association depends on Nse6 and specific sub-pathways, with implications for homologous recombination.

This study investigates HR-restart of replication forks using polymerase usage sequencing and Monte Carlo simulations in S. pombe, showing Pol δ synthesizes both strands without maturing to δ/ε and demonstrating uninterrupted fork progression through barriers.

The paper shows that disrupting dNTP homeostasis is synthetically lethal with CDK-induced replication stress in fission yeast, leading to genome instability and cell death.