Ashley M. Jacobi

UNCOVERseq is a workflow for sensitive off-target nomination in CRISPR-Cas editing, demonstrating superior performance across multiple modalities and systems.

PASTA quantifies chromosomal translocations from amplicon sequencing with high accuracy and sensitivity, outperforming existing methods in detecting rare rearrangements.

The paper analyzes CRISPR-Cas9 off-target mutations and translocations at single-cell and tissue-specific levels in mice, revealing context-dependent variability in editing outcomes.

This paper develops gene-edited tacrolimus-resistant Treg cells (FKBP12KO-Treg) for clinical use in transplant recipients, showing they maintain immunoregulatory function while resisting tacrolimus.

This study shows that silencing CCR2 in sensory neurons blocks bone cancer progression and alleviates pain by disrupting neuropeptide-driven tumor growth.

CRISPAltRations is a cloud-based tool that improves the analysis of CRISPR-induced DNA repair events by outperforming existing software in annotating indels and HDR events.