This paper introduces FPhaS, a framework for confidence-supported label-free metabolic imaging using phase autofluorescence microscopy, improving accuracy by integrating structural and metabolic data.

bioRxiv · Jun 17, 2026 · PreprintBioengineering

Two-photon activation, deactivation, and coherent control of melanopsin in live cells

Carlos A. Renteria, Jiho Kahng, Brian E. Tibble, Rishyashring R. Iyer, Jindou Shi, Haya A. AlGrain, Eric James Chaney, Edita Aksamitiene, Yuan-Zhi Liu, Phyllis R. Robinson, Tiffany M. Schmidt, Stephen A. Boppart

This paper demonstrates two-photon activation and modulation of melanopsin in live cells to study its tri-stability and function.

bioRxiv · Mar 26, 2025 · PreprintBioengineering

Cholesterol Metabolite 27-Hydroxycholesterol Enhances the Secretion of Cancer Promoting Extracellular Vesicles by a Mitochondrial ROS-Induced Impairment of Lysosomal Function

Anasuya Das Gupta, Jaena Park, Janet Elise Sorrells, Hannah Kim, Natalia Krawczyńska, Dhanya Pradeep, Yu Wang, Hashni Epa Vidana Gamage, Adam T. Nelczyk, Stephen A. Boppart, Marni D. Boppart, Erik Russell Nelson

This study shows that 27-hydroxycholesterol increases EV secretion by impairing lysosomal function through mitochondrial ROS, and antioxidant treatment reverses this effect.

Endocrinology · Sep 17, 2024Peer-reviewedCell Biology

Weakly supervised identification of microscopic human breast cancer-related optical signatures from normal-appearing breast tissue

Jindou Shi, Haohua Tu, Jaena Park, Marina Marjanović, Anna M. Higham, Natasha N. Luckey, Kimberly A. Cradock, Zifei Liu, Stephen A. Boppart

The paper introduces a weakly supervised deep learning framework to identify cancer-related optical signatures in normal-appearing breast tissue using SLAM imaging.

Biomedical Optics Express · Feb 16, 2023Peer-reviewedBioinformatics