Shan-Chi Hsieh

The paper identifies native T-DNA transfer systems in rhizobia that deliver cargo genes into legume nodules, showing they share similarities with Agrobacteria-mediated transformation but have distinct regulatory mechanisms.

The paper identifies diverse Tn7 family transposons in archaea, including Asgard groups, and shows they target specific sites like tRNA and ribonucleotide reductase genes.

The paper identifies telomeric transposons in cyanobacteria and Streptomyces that replace native telomeres and influence genome evolution.

The paper uses cryo-EM to reveal how TnsC regulates target-site selection in CRISPR-associated transposition systems by transferring polarity information to the transposase.

The paper shows that Tn7-like elements use Cas12K-based systems with truncated repeat-spacer units to target tRNA genes as attachment sites for transposition.

The paper shows Tn7-CRISPR-Cas elements use guide RNA categorization for dual targeting pathways, enabling chromosomal and plasmid transposition with selective regulation and privatization.