Christof Fellmann

The paper investigates DNA repair in postmitotic neurons and shows that manipulating repair pathways can influence CRISPR editing outcomes.

The paper uses a genetic minimization technique to identify minimal DNA-binding modules in Cas9, showing that large domain deletions can retain function.

Inserting theophylline aptamers into the sgRNA yields ligand-activated and ligand-deactivated guides that tune CRISPRi in E. coli over 11x to 13x dynamic ranges, enabling two-ligand multiplexing.