Gene editing of CD3 epsilon gene to redirect regulatory T cells for adoptive T cell transfer
Weijie Du, Fatih Noyan, Oliver McCallion, Vanessa Drosdek, Jonas Kath, Viktor Glaser, Carla Fuster‐García, Mingxing Yang, Maik Stein, Clemens Franke, Yaolin Pu, Olaf Weber, Julia K. Polansky, Toni Cathomen, Elmar Jaeckel, , Dimitrios L. Wagner
This paper develops a non-viral CRISPR-Cas12a method to redirect Tregs with CARs by integrating an antigen-binding domain into the CD3 epsilon gene, showing improved antigen-specific function and graft protection in a mouse model.
Molecular Therapy · Feb 3, 2025Peer-reviewedImmunology