Fadi Issa

ClonoDeconv estimates paired TCR clonotype proportions from bulk-TCR sequencing using a single-cell reference, capturing longitudinal T-cell clonal dynamics.

The paper develops a spatial tri-omics workflow to map genetically distinct myeloma subclones in bone marrow, showing they occupy spatially and biologically distinct niches.

This paper introduces ARMS DNAseq, a scalable spatial genomic assay that maps copy number subclones in archival tissue, revealing tumor evolution and subclone associations with morphology and transcriptomic features.

This paper develops gene-edited tacrolimus-resistant Treg cells (FKBP12KO-Treg) for clinical use in transplant recipients, showing they maintain immunoregulatory function while resisting tacrolimus.

This study uses spatial transcriptomic analysis and machine learning to redefine the topology of human bone marrow in health and myeloproliferative neoplasms, revealing new insights into cellular heterogeneity and fibrosis.

This paper develops a non-viral CRISPR-Cas12a method to redirect Tregs with CARs by integrating an antigen-binding domain into the CD3 epsilon gene, showing improved antigen-specific function and graft protection in a mouse model.