ImmunologyMolecular TherapyPeer-reviewed
Gene editing of CD3 epsilon gene to redirect regulatory T cells for adoptive T cell transfer
Weijie Du, Fatih Noyan, Oliver McCallion…·Dimitrios L. Wagner
This paper develops a non-viral CRISPR-Cas12a method to redirect Tregs with CARs by integrating an antigen-binding domain into the CD3 epsilon gene, showing improved antigen-specific function and graft protection in a mouse model.