Yiyun Rao

The paper presents a multimodal perturbation atlas using CRISPR knockouts in A549 cells, combining microscopy, phase imaging, and scRNA-seq to assess phenotypic resolution.

The paper validates that synonymous mutations in the human genome rarely affect fitness, occurring 165-fold less frequently than non-synonymous mutations.

The paper introduces MutSigCVsyn, an algorithm to identify synonymous cancer drivers by accounting for mutational heterogeneity, and identifies 30 novel synonymous drivers in cancer whole-genome sequences.

The paper identifies a common genetic architecture that explains context-specific gene essentiality across cell lines using CRISPR screens and machine learning.