Melissa Helen Little

This paper develops PT-enhanced kidney organoids with S3-like cells to model physiological responses in CKD, showing improved functionality and scalability.

This paper benchmarks spatial transcriptomics in stem cell-derived organoids using Stereo-seq, optimizing RNA capture and introducing regional analysis methods.

This study classifies off-target and indeterminate cell types in human kidney organoid differentiation using single-cell transcriptomics.

This study shows that hPSC-derived distal nephron epithelium can adopt ureteric or nephric duct-like identities in vitro in response to extracellular signals.

This study shows that distinct FGF subfamily members modulate nephron patterning in kidney organoids, with FGF8b and FGF10 having unique effects on proximal and distal nephron development.

This study generates integrated kidney assembloids by co-culturing nephron-forming and nephric duct progenitors, demonstrating functional connectivity between nephrons and a single collecting duct.

DevKidCC classifies kidney organoid cell subtypes accurately by comparing datasets to a reference model trained on human fetal kidney single-cell RNA-seq data.

The paper uses single-cell RNA-seq to analyze developing mouse kidney and human kidney organoids, identifying cell clusters and revealing insights into kidney development and organoid maturation.

This paper introduces an agent-based model of kidney branching morphogenesis and uses Bayesian inference to show that growth factor-dependent cell division is essential for branching.