Gerard Michael Coughlin

The paper establishes a method for in utero genome editing in fetal mouse cortex at midgestation, demonstrating efficient editing with reduced off-target effects.

The paper solves cryo-EM structures of AAV capsids and their complexes with AAVR to design variants with reduced liver targeting and altered brain cell tropism.

This paper introduces three HCR variants that improve sensitivity for detecting low-abundance mRNA targets in challenging tissue environments.

The paper identifies transcriptional crosstalk between enhancers and promoters delivered by AAVs, showing concatemer formation enables targeted delivery of large genetic cargo for cell-type-specific gene editing.

The paper introduces a programmable self-assembling protein system to model intracellular protein aggregation in neurodegenerative diseases and reveals disease-specific aggregate-organelle interactions.

The paper applies molecularly-guided single-cell spatial proteomics to study proteomic differences in healthy and diseased mammalian brains, revealing insights into Parkinson's disease.

The paper shows that timed activation of VIP neurons in the SCN and late afternoon light exposure can rescue dysregulated estrus cycles in mice.