Author

Arend W. Overeem

Leiden University Medical CenterORCID 0000-0003-2373-0252Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Developmental Biology· 2

Signature Assays, Organisms & Methods

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Research Focus:cell type annotation· 2 Research Focus:clustering· 2 Research Focus:differential expression· 2 Approach:experimental assay· 2 Organism:human· 2 Research Focus:cell-cell communication· 1 Assay:scRNA-seq· 1

Frequent Co-Authors

Susana M. Chuva de Sousa LopesiD· 2 shared Talia van der HelmiD· 2 shared Yolanda W. ChangiD· 2 shared Albert Blanch-AsensioiD· 1 shared Celine M. RoelseiD· 1 shared Christian Freund· 1 shared Hailiang MeiiD· 1 shared Ioannis MoustakasiD· 1 shared

Activity by Year

2022-2025 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 35 mutual co-author links

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2 shared papersDevelopmental Biology

Susana M. Chuva de Sousa LopesORCID

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2 papers

Recent·Most cited

Developmental BiologybioRxivPreprintMar 21, 2025

Reconstitution of human fetal ovaries reveals niche requirements for primordial germ cell-like cell progression

Yolanda W. Chang, Marjolein Trimp, Talia van der Helm…·Susana M. Chuva de Sousa Lopes

This study shows that human primordial germ cell-like cells can mature in reconstituted human fetal ovaries and amnion-like cell aggregates, with SCF being crucial for hPGCLC survival.

2 citations

humanscRNA-seq

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Developmental BiologyCell Reports MethodsPeer-reviewedJan 1, 2022

Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlay

Arend W. Overeem, Yolanda W. Chang, Ioannis Moustakas…·Susana M. Chuva de Sousa Lopes

This paper introduces a method to efficiently generate human primordial germ cell-like cells (hPGCLCs) in 2D culture using basement membrane extract overlay and low BMP4 concentration.

32 citations

humanexperimental assay

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