Andreas Houben

GRASP enables transformation-independent CRISPR-based chromatin isolation in plants by using dCas9-gRNA complexes to capture specific genomic regions from purified nuclei.

This study identifies two KNL2 genes in cowpea, characterizes their expression and subcellular localization, and examines their functional roles in centromere assembly and seed development.

The paper identifies genes and mechanisms involved in programmed chromosome elimination in Aegilops speltoides, focusing on B chromosome-encoded cohesin and centromere-related factors.

This study compares holocentromere organization in two Cyperaceae subfamilies, revealing differences in repeat structure and protein distribution associated with centromere function.

The paper identifies chromosomal and genetic variability in saffron crocus cultivars, revealing regional somaclonal lineages despite clonal origin.

This study identifies 28 candidate genes involved in B chromosome elimination in Sorghum purpureosericeum embryos through transcriptomic and genomic analysis.

The paper compares centromere organization and (epi)genome features between two species with different centromere types, finding distinct histone phosphorylation patterns and kinetochore gene mutations.

The paper introduces CRISPR-CISH, a chromogenic in situ DNA detection method that uses CRISPR/dCas9 to generate target-specific signals visible under bright-field microscopes.

This study shows that gene expression and epigenetic states remain stable after CRISPR/Cas-mediated chromosomal inversions in Arabidopsis thaliana.

The paper analyzes centromere structure and function in stick insects, revealing a functionally monocentric system with holocentric-like features and chromosome-specific centromeres.

The paper identifies repeat-based holocentromeres in Luzula sylvatica and proposes mechanisms for the evolutionary transition from monocentric to holocentric chromosomes.

This study shows that holocentric Cuscuta species have lost the standard kinetochore and SAC, with gene loss and protein mislocalization observed.

The paper analyzes sequence gaps in the barley MorexV3 reference genome, identifying centromeric and repeat regions as major contributors to fragmentation and discussing strategies for gap closure.

The paper proposes a model explaining how centromeric nucleosomes assemble into line-like holocentromeres during chromosome condensation using polymer simulations.

This study evaluates the effectiveness of target capture sequencing (TCS) for characterizing repetitive DNA in Rhynchospora species, showing reliable results comparable to genome skims.

The paper presents a chromosome-scale rye genome assembly to study genomic evolution and agronomic traits in Triticeae crops.

The paper investigates the functional roles of two paralogous CENH3 variants in cowpea centromeres, finding that CENH3.1 is essential for plant development and reproduction while CENH3.2 shows reduced functionality.

The paper reports high-quality genome sequences of European flint maize lines and analyzes genomic dynamics between European and US maize germplasm.

The paper investigates the mechanism of B chromosome drive in Aegilops speltoides, identifying nondisjunction and asymmetric spindles as key factors in their accumulation.