L. Peter Sarin

This study uses ribosome profiling to analyze translational dynamics between an archaeal virus and its host, revealing co-regulated gene expression and post-transcriptional regulation during infection.

This study maps stress-induced changes in yeast tRNA modifications, revealing stress-specific reprogramming and introducing a metric to separate modification changes from tRNA abundance.

This study uses human stem cells and tissues to investigate LCCS1, revealing impaired RNA metabolism and developmental defects in neural crest and neuromuscular systems.

The paper introduces a cost-effective reverse transcriptase, MarathonRT, and compares its performance with commercial enzymes for tRNA sequencing library preparation.

This study shows queuosine modification enhances phage resistance and biofilm formation in Shewanella glacialimarina by altering translation efficiency and promoting mutagenesis.

This study shows that queuosine modification in Shewanella glacialimarina is essential for cold-active growth, as its deficiency leads to cold sensitivity and metabolic changes.

The paper describes a rapid tRNA isolation method and a non-radioactive chemiluminescent Northern blotting technique for detecting tRNA and tRNA-derived fragments.

This paper describes a novel halophilic archaeon, Halogranum roseipondis, and its associated virus, Hagravirus capitaneum, which encodes an unusually high number of viral tRNA genes.

The paper introduces DepStep, a one-step rRNA depletion method for non-model organisms, which efficiently removes rRNA and reduces costs compared to commercial kits.

This paper develops an optimized protocol for producing and benchmarking MNaseA and MNaseB, showing MNaseA's superior activity in high-salt conditions for ribosome profiling in halophilic archaea.

This study identifies post-transcriptional modifications in Arabidopsis chloroplast tRNAs and rRNAs, revealing similarities to bacterial systems and unique features that may aid in codon discrimination and ribosome function.

The paper introduces a rapid UPLC-MS method for analyzing RNA modifications in complex samples, demonstrating its effectiveness across different organisms.

This paper develops a sensitive nLC-MS method using capillary porous graphitic carbon columns to quantify chemically modified ribonucleosides at attomol levels in complex samples.