Sebastian A. Leidel

The small ORF (smORF)-encoded polypeptide MIRINE promotes neuronal differentiation in vertebrate brain development

The paper identifies MIRINE, a small ORF-encoded polypeptide that promotes neuronal differentiation in vertebrate brain development by regulating the balance between proliferation and maturation.

Harmonized nucleoside mass spectrometry enables reproducible cross-platform RNA modification quantification

The paper benchmarks cross-platform RNA modification quantification using LC-MS/MS and finds that harmonized workflows and SILIS normalization improve reproducibility across different instrument types.

Loss of host factor-mediated m6Am methylation of the viral RNA cap impairs SARS CoV-2 replication

This study identifies PCIF1 as a host factor that methylates the SARS-CoV-2 RNA cap, enhancing viral replication and reducing disease severity in mice.

A dietary pan-amino acid dropout screen in vivo reveals a critical role for histidine in T-ALL

This study identifies histidine depletion as an antileukemic strategy in T-ALL by revealing its role in suppressing cholesterol biosynthesis through ribosome stalling and GCN2 activation.

tRNA thiolation defects disrupt cellular proteostasis and tissue homeostasis in mammals

This study shows that tRNA thiolation defects in humans cause proteostasis and tissue homeostasis issues, but mice with similar defects develop normally.

Pus7 mutation links tRNA dysregulation to aggressive behavior through activation of the integrated stress response and glycolytic reprogramming

The paper shows that Pus7 mutations reduce tRNA-Asp levels, activating the integrated stress response and glycolytic reprogramming, which contributes to aggressive behavior.

A rapid protocol for ribosome profiling of low input samples

This paper introduces a rapid ribosome profiling protocol for low-input samples, reducing RNA input to 0.1 pmol and enabling high-quality data from small samples.

The translation inhibitor cycloheximide affects ribosome profiling data in a species-specific manner

This study shows that cycloheximide affects ribosome profiling data differently in yeast and human cells, suggesting that CHX may not be necessary for accurate human ribosome profiling.

Estrogen receptor alpha controls gene expression via translational offsetting

This study shows estrogen receptor alpha (ER) regulates gene expression by coordinating transcription with translational offsetting, maintaining protein levels despite changes in mRNA abundance.

Attomol-level quantification of chemically modified ribonucleosides enabled by capillary porous graphitic carbon columns in nano LC-MS

This paper develops a sensitive nLC-MS method using capillary porous graphitic carbon columns to quantify chemically modified ribonucleosides at attomol levels in complex samples.