Kensuke Sakamoto

The paper uses cryo-EM to analyze endogenous mGluR2-containing receptor assemblies in mouse brain, revealing distinct structural states and complexes not previously observed in recombinant systems.

The paper investigates whether mGluR2 and 5-HT2A receptors physically interact in vitro or in vivo and finds no evidence for direct interaction or oligomerization.

The paper tests if molecules from ultra-large libraries are more selective than those from bio-like libraries, finding similar off-target activity despite differences in chemical novelty.

This study investigates the polypharmacological profiles of xanomeline and its metabolite N-desmethylxanomeline, revealing their agonist and antagonist actions at multiple biogenic amine G protein-coupled receptors.

This paper presents computational methods for designing GPCR-binding miniproteins and validates them through high-throughput screening, producing agonists and antagonists for multiple GPCRs.

This study evaluates AlphaFold2 models for ligand discovery, finding they perform similarly to experimental structures despite conformational differences, and identify potent agonists for the 5-HT2A receptor.