Molly J. Gasperini

A prime-editing recorder in one mouse embryo yields a dated, zygote-rooted lineage tree of 1.28M cells at E13.5, enabling clonal dominance, sibling-fate and cell-type coupling analyses.

The paper presents a barcoded multiplex strategy to screen enhancer AAVs using single-cell RNA sequencing and taxonomy mapping, but finds significant technical and biological noise complicates accurate expression pattern analysis.

The paper develops a toolkit of transgenic mouse lines and enhancer AAVs for selective targeting of cortical cell populations using transcriptomic and epigenomic data.

The paper uses a combined single-cell genetic and chemical screening platform to analyze kinase dependencies in glioblastoma response to targeted therapy, identifying adaptive signaling and potential combination therapies.

The paper functionally classifies thousands of BRCA1 variants using saturation genome editing to determine their impact on cancer risk.

This paper introduces crisprQTL mapping to identify gene regulation by using CRISPR perturbations and single-cell RNA-seq in human cells, finding 128 cis-regulatory elements affecting nearby genes.

The paper identifies a major issue with CRISPR-based single cell screens due to guide-barcode swapping and presents an optimized method to improve accuracy.

This paper introduces ScanDel, a CRISPR-based method to systematically delete non-coding regions of a gene locus, revealing that distal regulatory elements are not critical for HPRT1 function.