Joseph S. Beckwith

This paper introduces a new spectral single-molecule microscopy technique using spatially patterned detectors, achieving spectral and positional resolution without complex optical components.

This paper introduces a method combining single-molecule light-field microscopy with widefield Fourier light-field microscopy to improve volumetric subcellular imaging and molecular diffusion analysis.

This paper introduces ASA-PD, an imaging method to detect and map α-synuclein oligomers in Parkinson's disease brain tissue, revealing a specific subpopulation of nanoscale oligomers as an early hallmark of the disease.

The paper identifies a cellular tipping point between aggregate accumulation and removal as a universal feature of neurodegeneration, validated through live-cell assays and super-resolution imaging.

The paper identifies microglial activation and alpha-synuclein oligomers as drivers of early neuroinflammation in Parkinson's disease using transcriptomic and aggregate profiling in early-stage brain samples.

The paper maps α-synuclein oligomers in Parkinson's disease brain tissue to identify aggregation-susceptible cells and proposes a population-level model for early pathology.

RASP detects fluorescent puncta in complex cellular backgrounds with high precision and speed, outperforming existing methods by using image gradients to separate objects from noise.