Steven F. Lee

This paper introduces a new spectral single-molecule microscopy technique using spatially patterned detectors, achieving spectral and positional resolution without complex optical components.

This paper introduces patch deconvolution to accelerate 3D cellular imaging in Fourier light-field microscopy, achieving faster reconstruction speeds without compromising quality.

This paper introduces a method combining single-molecule light-field microscopy with widefield Fourier light-field microscopy to improve volumetric subcellular imaging and molecular diffusion analysis.

The paper tracks SOX2 and NANOG dynamics in live cells using advanced microscopy and analysis pipelines, revealing differences in their spatiotemporal behavior due to protein-protein interactions.

This paper introduces SMOLM-LFM, a method for 3D single-molecule orientation and localization microscopy using ratiometric intensity estimation without polarization projections, enabling 6D molecular analysis.

This paper introduces a contrastive learning framework for nanoscale spatial-omics, enabling analysis of single-molecule localisation data to study protein architecture and its changes under various conditions.

This paper introduces ASA-PD, an imaging method to detect and map α-synuclein oligomers in Parkinson's disease brain tissue, revealing a specific subpopulation of nanoscale oligomers as an early hallmark of the disease.

Nano-org provides a curated resource of single-molecule localisation microscopy data for studying protein nanoscale distributions and their relationships across cell types and conditions.

The paper identifies a cellular tipping point between aggregate accumulation and removal as a universal feature of neurodegeneration, validated through live-cell assays and super-resolution imaging.

The paper shows that T-cell activation is initiated by partial CD45 exclusion at sub-micron contacts, leading to strong activation and cytotoxicity through small signaling changes.

The paper identifies microglial activation and alpha-synuclein oligomers as drivers of early neuroinflammation in Parkinson's disease using transcriptomic and aggregate profiling in early-stage brain samples.

The paper maps α-synuclein oligomers in Parkinson's disease brain tissue to identify aggregation-susceptible cells and proposes a population-level model for early pathology.

The paper quantifies proteasome re-organization during proteotoxic stress using single-molecule microscopy and shows cytoskeleton-dependent movement restrictions.

This study uses 3D super-resolution microscopy to analyze PSD95 nanoarchitecture in the mouse brain, revealing diffuse supercomplexes outside synapses and distinct subtypes within synapses.

This paper introduces vortex light field microscopy, achieving 3D spectral single-molecule imaging with 25 nm spatial and 3 nm spectral precision.

RASP detects fluorescent puncta in complex cellular backgrounds with high precision and speed, outperforming existing methods by using image gradients to separate objects from noise.