Kevin Takasaki

The paper characterizes the 3D organization of large axons in human white matter, revealing distinct architectural patterns across different brain regions.

The paper introduces ExA-SPIM, a microscope that enables high-resolution imaging of large tissue samples without sectioning, demonstrating its use on mouse brains and human white matter.

The paper identifies neural assemblies consistent with Hebb's theory using a novel dataset linking optical physiology to connectivity, finding they are structurally integrated but not defined by strong monosynaptic connections.

The paper introduces a computational pipeline for automated tracing and assembly of axons across serial sections, validated on centimeter-scale data for orientation analysis and proofreading.

The paper shows that impaired capillary-venous drainage in aging mice leads to hypoperfusion, gliosis, and demyelination in deep white matter regions.

The paper visualizes dense distributions of immunolabeled proteins in adult macaque visual cortex using expansion microscopy and light sheet imaging, enabling multimodal atlas creation.

The paper shows motion/direction-sensitive thalamic neurons project more to middle layers of V1 than previously thought, challenging existing views on thalamocortical organization.

The paper determines the depth limit of 2-photon imaging in mouse brain tissue by comparing 2- and 3-photon excitation, finding accuracy ceases at 450 m where in- and out-of-focus fluorescence balance.

The paper maps the functional and structural properties of feedforward projections from the LGN to V1 in mice, revealing differences in receptive fields and depth-dependent biases between location-sensitive and motion-sensitive axons.

This paper introduces dual-plane 3-photon microscopy with remote focusing, enabling faster in vivo calcium imaging of two cortical planes in mice.