The paper develops SPARTA for in situ genetic reprogramming of native microbiomes and introduces ScRibe-seq to identify engineered taxa, demonstrating functionality across diverse environments.

bioRxiv · Nov 10, 2026 · PreprintBioengineering

Rapid in vitro synthesis of DNA templates via Sidewinder for polyadenylated Hantavirus mRNA vaccine candidates

Ezri Abraham, Joel Andrade, Alexander Davis, Tomasz Gawda, Jacob Glanville, Daniel Graves, Jianyi Huang, Jeongsoo Hur, Sangil Kim, Jean-Sebastien Paul, Noah Evan Robinson, Charles Sanfiorenzo, Sixiang Wang, Raymond J. Zhang, Weilin Zhang, , Kaihang Wang

This study develops a rapid DNA template synthesis method called Sidewinder to produce polyadenylated Hantavirus mRNA vaccine candidates in just 2 days.

bioRxiv · May 25, 2026 · PreprintBiochemistry

AAVs Targeting Human Carbonic Anhydrase IV Enhance Gene Delivery to the Brain

Changfan Lin, Xinhong Chen, Jonathan D. Hoang, Fiona Ristic, Yujie Fan, Seongmin Jang, Jin Hyung Alex Chung, Erin E. Sullivan, Tomasz Gawda, Bill Kavvathas, Irene Tran, Yitong Li, Andrew D. Steele, Timothy F. Shay, Viviana Gradinaru

This study engineers AAVs targeting human carbonic anhydrase IV to enhance brain gene delivery, finding that optimized variants achieve 100-fold greater transduction than AAV9.

Cell Reports · Oct 31, 2025Peer-reviewedBioengineering