The paper uses long-read RNA-seq to analyze temporal transcriptional dynamics in single medfly embryos, identifying novel genes and developmental transitions.

bioRxiv · Oct 29, 2025 · PreprintGenomics

Genome assembly of five Tephritid species for the enhancement of the Sterile Insect Technique

Haig Djambazian, Shu‐Huang Chen, Pierre Bérubé, Katerina Nikolouli, Maria‐Eleni Gregoriou, Dimitris Rallis, Alistair C. Darby, Nikolai Windbichler, Marc F. Schetelig, Philippos Aris Papathanos, Kostas D. Mathiopoulos, Gur Pines, Jiannis Ragoussis, Kostas Bourtzis

The paper presents chromosome-level genome assemblies for five Tephritid species using long-read sequencing, short-read polishing, and Hi-C scaffolding.

bioRxiv · Sep 15, 2025 · PreprintEcology

CRISPR/Cas9-mediated mutagenesis of the white-eye gene in the tephritid pest Bactrocera zonata

Albert Nazarov, Tamir Partosh, Flávia Krsticevic, Dimitris Rallis, Yael Arien, Guy Ostrovsky, Reut Madar Kramer, Eyal Halon, Alfred M. Handler, Simon W. Baxter, Yoav Gazit, Kostas D. Mathiopoulos, Gur Pines, Philippos Aris Papathanos

The paper develops a genetic tool for Bactrocera zonata by creating a chromosome-level genome and CRISPR/Cas9-induced white-eye mutants to aid in Genetic Sexing Strain development.

Insect Science · Aug 29, 2025Peer-reviewedBioengineering

Detection of sex chromosomes in Tephritid pests using R-CQ and KAMY, two computational methods to enable species specific control.

Dimitris Rallis, Konstantina T. Tsoumani, Flávia Krsticevic, Philippos Aris Papathanos, Georgia Gouvi, Angela Meccariello, Kostas D. Mathiopoulos, Alexie Papanicolaou

The paper introduces two computational methods, R-CQ and KAMY, to detect sex chromosome sequences in Tephritid pests and evaluates their performance on chromosome-level assemblies.

bioRxiv · Oct 27, 2023 · PreprintGenomics