Philippos Aris Papathanos

This study identifies cholesterol and desmosterol metabolism as critical for mosquito development and reproduction, revealing a diet-encoded regulatory mechanism and a metabolic vulnerability in mosquitoes.

The paper identifies the Y-linked gene draupnir in Anopheles gambiae, showing it escapes transcriptional silencing during spermatogenesis and is necessary for Y-chromosome-based sex-ratio distortion.

The paper compares outcomes of X-poisoning and X-shredding in malaria mosquitoes by timing Cas9 expression during spermatogenesis, finding that timing determines distortion type.

This study identifies a fluorescent marker that disrupts female blood-feeding and shortens lifespan in Anopheles gambiae, highlighting the importance of validating transgenic components.

This paper develops a genetic sexing strain in Aedes albopictus mosquitoes by combining CRISPR-engineered selectable traits with sex conversion, enabling efficient and accurate sex separation.

YOLito automates mosquito detection and behavioral analysis, achieving high accuracy across species and assays.

The paper introduces 3D-Feedy, an open-source membrane feeder for mosquitoes that is cost-effective, portable, and achieves comparable or better performance than commercial alternatives.

The paper tests a Y-linked Cas9 expression system in Drosophila melanogaster and finds reduced Cas9 activity, suggesting meiotic sex chromosome inactivation.

The paper presents chromosome-level genome assemblies for five Tephritid species using long-read sequencing, short-read polishing, and Hi-C scaffolding.

The paper develops a genetic tool for Bactrocera zonata by creating a chromosome-level genome and CRISPR/Cas9-induced white-eye mutants to aid in Genetic Sexing Strain development.

This study investigates chemosensory mechanisms in mosquito larvae, identifying functional variation in odorant receptors and mapping sensory neuron projections to understand competitive advantages in invasive species.

This study investigates X-poisoning gene drives in mosquitoes, finding that sex distortion is mainly due to loss of X-bearing sperm rather than postzygotic lethality.

This paper demonstrates the feasibility of using CRISPR-Cas9 gene drives in the medfly to achieve sex conversion and female sterility, offering a strategy for pest population suppression.

The paper introduces two computational methods, R-CQ and KAMY, to detect sex chromosome sequences in Tephritid pests and evaluates their performance on chromosome-level assemblies.

The paper analyzes the potential for an autosomal X-shredder to become a Y-linked drive element in malaria mosquitoes and concludes it is unlikely due to suppression mechanisms.

The paper engineers sex distortion in Ceratitis capitata using CRISPR/Cas9 and Cas12a to bias sex ratios towards males.

The paper presents an improved genome assembly of Aedes albopictus, revealing new biological features and enabling population and developmental studies.

The paper establishes Drosophila models to study two CRISPR/Cas9 sex distortion mechanisms, X-shredding and X-meddling, and their functional differences.

The paper identifies a Y-linked gene, MoY, essential for male sex determination in medflies and shows its conservation across multiple fruit fly species, enabling potential genetic control strategies.

This study evaluates synthetic reproductive barriers in Drosophila using CRISPR and CRISPRa, finding that synthetic lethality is achievable but with trade-offs in genetic isolation and fly fitness.

This paper evaluates Integral Gene Drive (IGD) as a more durable and flexible alternative to classical gene drives for population replacement, showing reduced resistance and prolonged transmission blockage.

Redkmer identifies X-chromosome specific sequences from raw WGS data to guide CRISPR-based sex ratio distortion in insects.

The paper investigates the evolutionary dynamics of sex-biased genes in four Anopheles mosquito species, finding female-biased genes evolve faster than male-biased ones due to sex-specific challenges.

The paper demonstrates that Y chromosomes from two malaria vector species in the Anopheles gambiae complex function interchangeably, suggesting no hybrid incompatibility loci on the Y and enabling potential cross-species sex-distorting Y drive interventions.