Lilia Evgeniou

TIGRFinder annotated 6,685 TIGR arrays; cryo-EM structures show inactive RuvC domains, and E. coli reporter assays show CsTasR and MbTasA repress transcription in a guide-dependent manner.

This study adapts Edman degradation and amino acid recognition to expansion microscopy gels to demonstrate in situ protein sequencing principles using synthetic constructs.

The paper designs and evaluates recombinase-based genetic programs in human cells that simulate a for-loop counting to three, demonstrating heritable memory and sequential gene expression.